LOCUS       40924_26461       10621 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pK18msr DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10621)
  AUTHORS   Schafer A, Tauch A, Jager W, Kalinowski J, Thierbach G, Puhler A.
  TITLE     Small mobilizable multi-purpose cloning vectors derived from the 
            Escherichia coli plasmids pK18 and pK19: selection of defined 
            deletions in the chromosome of Corynebacterium glutamicum
  JOURNAL   Gene 145 (1), 69-73 (1994)
  PUBMED    8045426
REFERENCE   2  (bases 1 to 10621)
  AUTHORS   Yamamoto S, Uraji M, Tanaka K, Moriguchi K, Suzuki K.
  TITLE     Identification of pTi-SAKURA DNA region conferring enhancement of 
            plasmid incompatibility and stability
  JOURNAL   Genes Genet. Syst. 82 (3), 197-206 (2007)
  PUBMED    17660690
REFERENCE   3  (bases 1 to 10621)
  AUTHORS   Yamamoto S, Suzuki K.
  TITLE     Development of a reinforced Ti-eviction plasmid useful for 
            construction of Ti plasmid-free Agrobacterium strains
  JOURNAL   J. Microbiol. Methods 89 (1), 53-56 (2012)
  PUBMED    22326751
REFERENCE   4  (bases 1 to 10621)
  AUTHORS   Yamamoto S, Suzuki K.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2012) Contact:Shinji Yamamoto Hiroshima 
            University, Graduate School of Science; Kagamiyama 1-3-1, 
            Higashi-Hiroshima, Hiroshima 739-8526, Japan
REFERENCE   5  (bases 1 to 10621)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 10621)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1994"; volume: "145"; issue: "1"; pages: "69-73"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Genes 
            Genet. Syst."; date: "2007"; volume: "82"; issue: "3"; pages: 
            "197-206"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "J. 
            Microbiol. Methods"; date: "2012"; volume: "89"; issue: "1"; pages: 
            "53-56"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Submitted 
            (23-JAN-2012) Contact:Shinji Yamamoto Hiroshima University, Graduate
            School of Science; Kagamiyama 1-3-1, Higashi-Hiroshima, Hiroshima 
            739-8526, Japan"
COMMENT     SGRef: number: 5; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             346..1137
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        1186..1631
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             1632..3050
                     /codon_start=1
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVSEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     oriT            complement(3407..3516)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     promoter        complement(3751..3841)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     promoter        complement(4035..4125)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      4485..5073
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5361..5382
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5397..5427
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5435..5451
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5459..5475
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5484..5540
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     protein_bind    5645..5658
                     /label=TraR binding site
                     /bound_moiety="TraR"
                     /note="Tra box"
     protein_bind    5736..5749
                     /label=TraR binding site
                     /bound_moiety="TraR"
                     /note="Tra box"
     CDS             5948..7165
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A"
                     /function="involved in vegetative DNA replication"
                     /label=repA
                     /protein_id="BAL46515.1"
                     /translation="MSKAAAISRNDRPSVDVTIGEHAEQLSSQLQAMSEALFPPTSHKS
                     LRKFTSGEAARLMKISDSTLRKMTLAGEGPQPELASNGRRFYTLGQINEIRQMLAGSTR
                     GRESIDFVPHRRGSEHLQVVAVTNFKGGSGKTTTSAHLAQYLALQGYRVLAVDLDPQAS
                     LSALLGVLPETDVGANETLYAAIRYDDTRRPLRDVIRPTYFDGLHLVPGNLELMEFEHT
                     TPKALTDKGTRDGLFFTRVAQAFDEVADDYDVVVIDCPPQLGFLTLSGLCAATSMVITV
                     HPQMLDIASMSQFLLMTRDLLGVVKEAGGNLQYDFIRYLLTRYEPQDAPQTKVTALLRN
                     MFEDHVLTNPMVKSAAVSDAGLTKQTLYEIGRENLTRSTYDRAMESLDAVNSEIEALIK
                     MAWGRV"
     gene            5948..7165
                     /gene="repA"
                     /label=repA
     CDS             7395..8405
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B"
                     /function="involved in vegetative DNA replication"
                     /label=repB
                     /protein_id="BAL46516.1"
                     /translation="MSRKDAIDTLFLKKQPATDRAAVDKSTARVRTGAISAMGSSLQEM
                     AEGAKAAARLQDQLATGEAVVSLDPSMIDGSPIADRLPSDVDPKFEQLEASISQEGQQV
                     PVLVRPHPEAAGRYQIVYGRRRLRAAVNLRREVSAIVRNLTDCELVVAQGRENLNRADL
                     SFIEKALFALRLEDAGFDRATIIAALSTDKADLSRYITVARGIPLNLATQIGPASKAGR
                     SRWVALAEGLGKPKATDAIEAMLGSEQFKQSDSDTRFNLIFNAVSRPPAKTPKKVRAWS
                     TPKGKKAATIRQETGRTALVFDERLVPTFGEYVADQLDSLYAQFIETNGGGKLDQ"
     gene            7395..8405
                     /gene="repB"
                     /label=repB
     rep_origin      8460..8618
                     /label=pTi-SAKURA oriV
                     /note="pTi-SAKURA oriV"
     CDS             8620..9939
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C"
                     /function="involved in vegetative DNA replication"
                     /label=repC
                     /protein_id="BAL46517.1"
                     /translation="MQTHISTTSFGRRPMTLGHIASQMAAKAVASDTVAHKWQVFQHIR
                     ESRGLIGATDRSLSILNALLTFYPETALTGGAELVVWPSNEQLMARANGMPATTLRRHL
                     AILVDCGLIIRRDSPNGKRFARKGRGGEIEQAYGFDLSPIVARAEEFRDLAQTVQAEKK
                     AFRVAKERLTLLRRDIVKMIETGVEESVPGNWGRVTQTYQGIIGRLPRSAPRQLVESIG
                     QELQELCIEIRDVLESFTKTMNLDANESHIGRHKQNSNPDSKFESEYGSGKKDEAGGSV
                     AETDNVRSLPKRELPLGIVLDACPEMRELAQGGPIRHWRDLLAAAELARPMLGISPSAW
                     REARETMGEQHAAITLASIYQRAGQINNAGGYLRSLTDRAKDGKFSTWPMVMALLRAKL
                     DEQKNAVGAGKPRTAEEVEDDSRLHVSESLLKNLRKPRSW"
     gene            8620..9939
                     /gene="repC"
                     /label=repC
     primer_bind     complement(10446..10462)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgccgcaagc actcagggcg caagggctgc taaaggaagc ggaacacgta gaaagccagt
       61 ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa
      121 aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac
      181 tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag
      241 gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc
      301 aggggatcaa gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
      361 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
      421 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
      481 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactccaaga cgaggcagcg
      541 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
      601 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
      661 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
      721 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
      781 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
      841 gcgccagccg aactgttcgc caggctcaag gcgcggatgc ccgacggcga ggatctcgtc
      901 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
      961 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     1021 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     1081 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     1141 gcgggactct ggggttcgct agaggatcga tcctttttaa cccatcacat atacctgccg
     1201 ttcactatta tttagtgaaa tgagatatta tgatattttc tgaattgtga ttaaaaaggc
     1261 aactttatgc ccatgcaaca gaaactataa aaaatacaga gaatgaaaag aaacagatag
     1321 attttttagt tctttaggcc cgtagtctgc aaatcctttt atgattttct atcaaacaaa
     1381 agaggaaaat agaccagttg caatccaaac gagagtctaa tagaatgagg tcgaaaagta
     1441 aatcgcgcgg gtttgttact gataaagcag gcaagaccta aaatgtgtaa agggcaaagt
     1501 gtatactttg gcgtcacccc ttacatattt taggtctttt tttattgtgc gtaactaact
     1561 tgccatcttc aaacaggagg gctggaagaa gcagaccgct aacacagtac ataaaaaagg
     1621 agacatgaac gatgaacatc aaaaagtttg caaaacaagc aacagtatta acctttacta
     1681 ccgcactgct ggcaggaggc gcaactcaag cgtttgcgaa agaaacgaac caaaagccat
     1741 ataaggaaac atacggcatt tcccatatta cacgccatga tatgctgcaa atccctgaac
     1801 agcaaaaaaa tgaaaaatat caagtttctg aatttgattc gtccacaatt aaaaatatct
     1861 cttctgcaaa aggcctggac gtttgggaca gctggccatt acaaaacgct gacggcactg
     1921 tcgcaaacta tcacggctac cacatcgtct ttgcattagc cggagatcct aaaaatgcgg
     1981 atgacacatc gatttacatg ttctatcaaa aagtcggcga aacttctatt gacagctgga
     2041 aaaacgctgg ccgcgtcttt aaagacagcg acaaattcga tgcaaatgat tctatcctaa
     2101 aagaccaaac acaagaatgg tcaggttcag ccacatttac atctgacgga aaaatccgtt
     2161 tattctacac tgatttctcc ggtaaacatt acggcaaaca aacactgaca actgcacaag
     2221 ttaacgtatc agcatcagac agctctttga acatcaacgg tgtagaggat tataaatcaa
     2281 tctttgacgg tgacggaaaa acgtatcaaa atgtacagca gttcatcgat gaaggcaact
     2341 acagctcagg cgacaaccat acgctgagag atcctcacta cgtagaagat aaaggccaca
     2401 aatacttagt atttgaagca aacactggaa ctgaagatgg ctaccaaggc gaagaatctt
     2461 tatttaacaa agcatactat ggcaaaagca catcattctt ccgtcaagaa agtcaaaaac
     2521 ttctgcaaag cgataaaaaa cgcacggctg agttagcaaa cggcgctctc ggtatgattg
     2581 agctaaacga tgattacaca ctgaaaaaag tgatgaaacc gctgattgca tctaacacag
     2641 taacagatga aattgaacgc gcgaacgtct ttaaaatgaa cggcaaatgg tacctgttca
     2701 ctgactcccg cggatcaaaa atgacgattg acggcattac gtctaacgat atttacatgc
     2761 ttggttatgt ttctaattct ttaactggcc catacaagcc gctgaacaaa actggccttg
     2821 tgttaaaaat ggatcttgat cctaacgatg taacctttac ttactcacac ttcgctgtac
     2881 ctcaagcgaa aggaaacaat gtcgtgatta caagctatat gacaaacaga ggattctacg
     2941 cagacaaaca atcaacgttt gcgccgagct tcctgctgaa catcaaaggc aagaaaacat
     3001 ctgttgtcaa agacagcatc cttgaacaag gacaattaac agttaacaaa taaaaacgca
     3061 aaagaaaatg ccgatgggta ccgagcgaaa tgaccgacca agcgacgccc aacctgccat
     3121 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     3181 gggacgccct cgcggacgtg ctcatagtcc acgacgcccg tgattttgta gccctggccg
     3241 acggccagca ggtaggccga caggctcatg ccggccgccg ccgccttttc ctcaatcgct
     3301 cttcgttcgt ctggaaggca gtacaccttg ataggtgggc tgcccttcct ggttggcttg
     3361 gtttcatcag ccatccgctt gccctcatct gttacgccgg cggtagccgg ccagcctcgc
     3421 agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac
     3481 ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa
     3541 ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat
     3601 accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa gcgctgcttc
     3661 cctgctgttt tgtggaatat ctaccgactg gaaacaggca aatgcaggaa attactgaac
     3721 tgaggggaca ggcgagagac gatgccaaag agctcctgaa aatctcgata actcaaaaaa
     3781 tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc
     3841 aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg
     3901 atttatttat tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc
     3961 gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg
     4021 cttctgtttc tatcagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     4081 cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc
     4141 caaaagttgg cccagggctt cccggtatca acagggacac caggatttat ttattctgcg
     4201 aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg cgtcgggtga tgctgccaac
     4261 ttactgattt agtgtatgat ggtgtttttg aggtgctcca gtggcttctg tttctatcag
     4321 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaaaagg
     4381 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     4441 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4501 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4561 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     4621 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     4681 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     4741 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     4801 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     4861 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     4921 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     4981 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5041 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5101 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     5161 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     5221 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     5281 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     5341 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     5401 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     5461 ggaaacagct atgacatgat tacgaattcg agctcggtac ccggggatcc tctagagtcg
     5521 acctgcaggc atgcaagctt cgataccgtt cgtattggtc cggcgaaact gtgagtatcc
     5581 gcatcgtaat ctccgcatga acaggtcatg cgaacagaaa tcatctcacg gtgcgtttgc
     5641 ctacgtgcag atttgcacct caggtgattc taccgagtcg gtgttccaag gcgcgaaatg
     5701 cgagcgggtg aggccggcca gacgccgaca aggttgtgca gatctgcact tggtgccacg
     5761 tcgcacagaa gaagggaatc ggtctaactc acagatagca tttgaagaat cgggatttag
     5821 tgtgatttcg attgaaacgc gcgtaaccgt tcattaacca aaaacgtctt gcaacctcac
     5881 ccgcattagg taatcgtcac ggataaatgg caatacgcgc caattaaccg tgacaagaga
     5941 taacaccgtg agcaaagccg ctgccatatc ccgaaatgat cgcccgtcgg tagatgttac
     6001 cattggtgag catgctgagc agctcagctc tcagcttcaa gcgatgagcg aggctttgtt
     6061 tcctccgacg tcgcacaaga gcttgcgcaa attcacctcg ggtgaagccg cacgcttgat
     6121 gaaaatatct gactcaactc ttcgaaagat gacactggct ggcgaagggc cgcaacctga
     6181 actcgccagc aacggacggc gcttttacac cctcggtcag ataaacgaaa tccggcagat
     6241 gcttgccggc tcgactcgag gacgtgaaag cattgatttt gtgcctcatc gccgaggttc
     6301 tgagcatttg caagtcgttg ctgtaaccaa cttcaaaggt ggctctggga agacgacgac
     6361 gtccgctcat cttgcacagt atctggcgtt gcaaggttac agggttctcg cagtcgatct
     6421 cgatccgcag gctagtcttt cagcactcct cggcgttctg ccagaaactg atgtcggtgc
     6481 aaacgaaacg ctctatgcgg ctattcggta cgacgacaca cgtcgtccgt tgcgagatgt
     6541 gatccgaccg acgtattttg atggtcttca ccttgttcct ggaaatctcg agcttatgga
     6601 gttcgagcat accaccccga aagcattgac tgacaaaggt acgcgcgacg gattgttctt
     6661 cactcgcgtg gcccaagcct ttgatgaggt cgccgacgat tacgatgtcg tggtcatcga
     6721 ctgccctcct cagcttgggt ttttgactct cagcgggttg tgtgctgcaa catcaatggt
     6781 aatcaccgta catcctcaga tgctggatat cgcttccatg agccagtttc tcctcatgac
     6841 acgcgacctt ctgggtgtcg tgaaagaggc ggggggcaat ctccagtacg atttcatacg
     6901 ctatctcttg acgcgctatg agccccagga cgcgccgcag acgaaagtga cggcactgct
     6961 gcgcaacatg ttcgaggatc acgtccttac aaatcctatg gtcaagtcgg cagcggtatc
     7021 tgatgccggt ttaaccaagc agacgctcta tgagataggg cgagagaacc ttacgcgatc
     7081 gacatacgac cgggcgatgg aatctttaga tgcggtgaat tcggagatcg aggctttgat
     7141 caagatggcg tgggggcggg tctaatgaaa ggctttgcgt tcctcacaga tctgttggga
     7201 gctcccaaca gacaggtgtt gattcgcccc ctggacatgg ggcactggag aagccggggt
     7261 aatttgagac gacgacgcac gcccatcgct aattggccag ggtgcagttg tcttgtcttg
     7321 ttgggagctc ccaaccaagc gcatttgcaa tcaaaaatgc gacgccacga cgccaaaccc
     7381 aagaggctga tatcatgagc cgcaaagacg caatcgatac tttgttcctc aagaagcaac
     7441 ctgcgaccga tagagcagca gtcgacaagt cgaccgctcg tgttcgtacc ggagcgattt
     7501 cggccatggg ttcgtctttg caagagatgg ctgagggcgc aaaggctgca gctcggctgc
     7561 aggatcaact ggctacaggc gaagccgtcg tgtccctgga tccatccatg atcgacgggt
     7621 cgccgatcgc ggatcggctg ccctcagacg tggatccgaa attcgagcag cttgaggcga
     7681 gcatttcgca ggaggggcag caggtgccgg ttcttgtcag accgcaccct gaggctgccg
     7741 gtcgatatca gatcgtatat ggaaggcggc ggctgcgcgc ggcagtaaat ctgcggagag
     7801 aggtttctgc cattgttcga aatctcacgg actgtgaact ggtcgtggcc cagggccgcg
     7861 aaaatcttaa ccgtgctgac ctctcgttca ttgagaaggc tctcttcgcc ctgcgcctcg
     7921 aagatgcggg ttttgataga gccaccatca ttgccgcgct atccactgac aaggccgacc
     7981 tcagccgcta cataactgta gcaaggggca taccgctgaa cctcgccaca caaatcggcc
     8041 cagcgtcgaa agcgggtcga tcgcgttggg tcgcacttgc cgaggggctt gggaagccta
     8101 aggcaacgga cgcaatcgaa gcgatgcttg ggtcagagca gttcaagcaa tctgatagcg
     8161 atacccgctt taacctcatt ttcaacgccg tttcaaggcc acctgcgaag actccaaaaa
     8221 aggtaagggc ctggagcacg ccaaagggga aaaaggcagc gacgatccga caagaaactg
     8281 gacgaacggc gctggttttc gacgagagac tggtgccaac ttttggcgaa tatgtcgctg
     8341 accagttgga cagtctgtac gcccagttca ttgaaaccaa cggaggaggc aagctcgacc
     8401 aatagtcagg gtttcatcca atttaaagct ccgctcgact gagatggact ggctctcacc
     8461 gcaaaagaaa aaggcccccg aaacggcgtt ccggaagacc ttctctgtag tctcgcagct
     8521 aagagaatcg catttccagg aatcgtagtc aagggtcccg taagggaaag cgtcatttcg
     8581 acgggcggat ttcaattgcc taacaaaagg taaaaggaaa tgcagacgca tatctcaacg
     8641 acgtcctttg ggcggcggcc gatgacactc ggccatattg caagccagat ggcagcaaaa
     8701 gcggtcgcat cagacactgt cgcccacaaa tggcaggtct tccagcacat ccgtgaatcc
     8761 cggggactga tcggagccac ggaccgctca ctctcgatcc tgaacgcgct gttgacgttt
     8821 tacccggaga ccgccttgac tggtggtgcc gaactggtcg tatggccttc taacgaacag
     8881 ctgatggctc gcgccaacgg catgcccgcc acgacactgc gccggcatct tgccatactg
     8941 gttgattgcg ggctcatcat tcgccgcgac agccccaatg gcaagcggtt cgcccgcaag
     9001 ggaaggggag gggagattga gcaggcctat gggttcgatc tgtcgccgat cgtcgcgcgg
     9061 gccgaggagt tccgagatct ggcccagaca gtgcaagctg aaaaaaaggc cttccgggtg
     9121 gccaaggagc gcttgactct tcttcgtcgt gacattgtca aaatgatcga aactggcgtc
     9181 gaagagagcg ttcctggaaa ctggggaaga gttacccaga cctatcaggg gatcatcggc
     9241 cgcctgccac gctcggcacc tcggcagctt gtcgagagta ttgggcaaga gcttcaggaa
     9301 ctttgcatcg agatccgtga cgtattggaa tctttcacaa aaacgatgaa tctggacgcc
     9361 aatgagtccc atatcggtcg ccacaaacag aattcaaatc cagactctaa atttgaatct
     9421 gaatacggct ctggaaaaaa agatgaagcg ggcggcagcg ttgcggaaac cgacaatgta
     9481 cggagcttgc cgaaacgcga gctgcctttg ggaatcgtgc tggatgcctg ccccgaaatg
     9541 cgggaattgg cccagggagg tccaattcga cattggcgcg acttgctggc ggcggctgag
     9601 cttgcccggc cgatgctggg gattagtccg agcgcctggc gggaggcccg cgaaaccatg
     9661 ggcgagcaac acgcggcgat cacgctggct tcgatctatc agcgggccgg tcagatcaat
     9721 aacgctgggg gctatctgcg cagcctgacc gaccgggcca aggatgggaa gttttcgacc
     9781 tggccgatgg tcatggcgtt gctccgggcg aagctggacg agcagaagaa tgcagtcggc
     9841 gctggaaagc cgcgaactgc tgaggaggtc gaggatgaca gccgcctcca cgtatcggaa
     9901 tcgctgctca aaaacctgcg aaagccgaga tcttggtgat cctctcgcta ttcagccgcg
     9961 gcgatgtcga cgtcggtgat caacccggaa cgtcgggcgc ggtcgatcag gttcttgacg
    10021 gacgagggcg cccatttgga tccaccgcgc ggcgtgcgct cgtgcagtct ttcaagctgg
    10081 ccggcgatct cgcggagctt caggtcgggg ttcgaagaat ggatgccggc cacaagcgtc
    10141 atcaggcgat cttcgggaag acggggagga gattttttca ggagcgctgc atccaccagg
    10201 cgttccgtca ccatccactt cacggcgcgg cgaagacgtt ccggcgtcca gtcgaagccc
    10261 cgctgcttga gcattcgggc gatgtcgtcc catgtgtgat ccggtcgcat gcgacgaacg
    10321 gtaggaagcc attggttcgc ggacgcctga atcctatcgc catatgccgc tttctgggcc
    10381 gcggtcatcc ttgccagtgc ctccgggcgc ttttcccgga tgcccgggtt gccggaaagc
    10441 ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
    10501 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
    10561 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgataagc tagcttcacg
    10621 c
//
